Are the individuals added in the owl file?
Owlready2 loads an owl file only one time from the disk and keeps it in memory.
When trying to load the same owl file again it will see it's already in memory and and just return it from there.
That's why you don't see any new modification that were made in the owl file on disk.
You can check how to avoid that here:
https://owlready2.readthedocs.io/en/latest/world.htmlHope this helps!